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ef1α promoter  (Addgene inc)


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    Structured Review

    Addgene inc ef1α promoter
    Ef1α Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcep4+oct4+wt/pCEP4_WT_OCT4+(Plasmid+%2340629)/pm40316310-176-12-17
    Average 93 stars, based on 7 article reviews
    ef1α promoter - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal dynamics in skin keratinocytes
    Article Snippet: The following antibodies were used: Oct4 (Cell Signaling, 2750) 1:1000, GAPDH (abcam, 9484) 1:2000, E-cadherin (ThermoFisher, PA5-32178) 1:5000, β-catenin (ThermoFisher, 13-8400) 1:1000, ZO1 (ThermoFisher, 33-9100) 1:300, β-actin (abcam, 8226) 1:1000, goat anti-rabbit DyLight-680 (ThermoFisher, 35568) 1:10000, goat anti-mouse DyLight-800 4X PEG (ThermoFisher, SA5-35521) 1:10000, donkey anti-goat DyLight-680 (ThermoFisher, SA5-10090) 1:10000. .. 1 × 10 6 HaCaT cells were seeded for 24 h. Then, cells were transfected with 5 μg of plasmid pcDNA3-EGFP (addgene, 13031), or pcDNA3.3-OCT4 (addgene, 26816), or pcDNA3-OCT4EGFP, or pCEP4-OCT4-WT (addgene, 40629), or pCEP4-OCT4-T234A-S235A (addgene, 40630) or pCEP4-OCT4-T234E-S235E (addgene, 40631) for 48 h using FuGENE 6 (Promega, E2691) according to the manufacturer’s protocol. .. For immunoprecipitation, 6 × 10 6 cells were seeded for 48 h. Untreated or transfected HaCaT cells were washed twice on ice with ice-cold 1X PBS, lysed in RIPA buffer (NaCl 150 mM, EDTA pH=8; 5 Mm, Tris-HCl pH=8; 50 mM, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS) supplemented with protease inhibitor tablets (ThermoFisher, A32955) for 15 min on ice and centrifuged at 4 °C for 15 min at 12.500 rpm.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: .. 1 x 106 HaCaT cells were seeded for 24 h. Then, cells were transfected with 5 mg of plasmid pcDNA3-EGFP (addgene, 13031), or pcDNA3.3-OCT4 (addgene, 26816), or pcDNA3-OCT4EGFP, or pCEP4-OCT4-WT (addgene, 40629), or pCEP4-OCT4-T234AS235A (addgene, 40630) or pCEP4-OCT4-T234E-S235E (addgene, 40631) for 48 h using FuGENE 6 (Promega, E2691) according to the manufacturer’s protocol. .. For immunoprecipitation, 6 x106 cells were seeded for 48 h. Untreated or transfected HaCaT cells were washed twice on ice with icecold 1X PBS, lysed in RIPA buffer (NaCl 150 mM, EDTA pH = 8; 5 Mm, Tris-HCl pH = 8; 50 mM, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1%SDS) supplementedwith protease inhibitor tablets (ThermoFisher, A32955) for 15min on ice and centrifuged at 4 C for 15 min at 12.500 rpm.

    Plasmid Preparation:

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal dynamics in skin keratinocytes
    Article Snippet: The following antibodies were used: Oct4 (Cell Signaling, 2750) 1:1000, GAPDH (abcam, 9484) 1:2000, E-cadherin (ThermoFisher, PA5-32178) 1:5000, β-catenin (ThermoFisher, 13-8400) 1:1000, ZO1 (ThermoFisher, 33-9100) 1:300, β-actin (abcam, 8226) 1:1000, goat anti-rabbit DyLight-680 (ThermoFisher, 35568) 1:10000, goat anti-mouse DyLight-800 4X PEG (ThermoFisher, SA5-35521) 1:10000, donkey anti-goat DyLight-680 (ThermoFisher, SA5-10090) 1:10000. .. 1 × 10 6 HaCaT cells were seeded for 24 h. Then, cells were transfected with 5 μg of plasmid pcDNA3-EGFP (addgene, 13031), or pcDNA3.3-OCT4 (addgene, 26816), or pcDNA3-OCT4EGFP, or pCEP4-OCT4-WT (addgene, 40629), or pCEP4-OCT4-T234A-S235A (addgene, 40630) or pCEP4-OCT4-T234E-S235E (addgene, 40631) for 48 h using FuGENE 6 (Promega, E2691) according to the manufacturer’s protocol. .. For immunoprecipitation, 6 × 10 6 cells were seeded for 48 h. Untreated or transfected HaCaT cells were washed twice on ice with ice-cold 1X PBS, lysed in RIPA buffer (NaCl 150 mM, EDTA pH=8; 5 Mm, Tris-HCl pH=8; 50 mM, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS) supplemented with protease inhibitor tablets (ThermoFisher, A32955) for 15 min on ice and centrifuged at 4 °C for 15 min at 12.500 rpm.

    Article Title: Oct4 is a gatekeeper of epithelial identity by regulating cytoskeletal organization in skin keratinocytes.
    Article Snippet: .. 1 x 106 HaCaT cells were seeded for 24 h. Then, cells were transfected with 5 mg of plasmid pcDNA3-EGFP (addgene, 13031), or pcDNA3.3-OCT4 (addgene, 26816), or pcDNA3-OCT4EGFP, or pCEP4-OCT4-WT (addgene, 40629), or pCEP4-OCT4-T234AS235A (addgene, 40630) or pCEP4-OCT4-T234E-S235E (addgene, 40631) for 48 h using FuGENE 6 (Promega, E2691) according to the manufacturer’s protocol. .. For immunoprecipitation, 6 x106 cells were seeded for 48 h. Untreated or transfected HaCaT cells were washed twice on ice with icecold 1X PBS, lysed in RIPA buffer (NaCl 150 mM, EDTA pH = 8; 5 Mm, Tris-HCl pH = 8; 50 mM, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1%SDS) supplementedwith protease inhibitor tablets (ThermoFisher, A32955) for 15min on ice and centrifuged at 4 C for 15 min at 12.500 rpm.



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